New generation culture media have an improved and complex composition that perfectly supports the embryo during its pre-implantation development in the laboratory. More specifically, for the prolonged culture for 5 or 6 days, up to the blastocyst stage, the so-called sequential culture media are used, as they have different components reflecting the continuously changing nutritional and metabolic requirements of the developing embryo.

Recently, new culture media have developed that contain special growth factors (e.g. GM-CSF) which seem to significantly benefit patients with previous miscarriages and possibly patients with previous failed assisted reproduction cycle attempts, poor sperm quality, etc.

The advances in the culture media field have contributed significantly to the increase in pregnancy rates.